Figure 4
The inhibitory effects of NC-8 on osteoclast bone resorption activity. (A and B) RAW 264.7 cells were treated with 100 ng/ml RANKL or 10 μg/ml NC-8 and cultured on a Corning Osteo Assay Surface culture plate. After RANKL treatment for 4 days and subsequent NC-8 treatment for 8 days, the area of resorption was observed under a 40 X microscope. The results are expressed as the mean ± S.E.M. of four independent experiments. *p < 0.05, **p < 0.005 compared with the control group.
