Figure 6
Effects of NC-8 on the OVX-induced up-regulation of serum markers for bone resorption. (A) NC-8 (1, 3 and 10 mg/kg each 3 days) significantly antagonized the OVX-induced increase of serum levels of osteoclastic markers. (B) NC-8 (1, 3 and 10 mg/kg each 3 days) also antagonized the compensatory increase of serum osteocalcin. Values represent the mean ± S.E.M. *p < 0.05 compared with the sham group (Sham). #p < 0.05 compared with the OVX group (OVX). (C) H&E staining shows that NC-8 (10 mg/kg each 3 days) antagonized OVX-induced trabecular bone loss. Black arrows indicate the cancellous bone in secondary spongiosa. (D) Bone-resorption parameters N.Oc/B.Pm and Oc.S/BS were quantified using OsteoMeasure image-analysis software. (NC-8: H: 10 mg/kg each 3 days) *p < 0.05, compared with the sham group (Sham). #p < 0.05 compared with the OVX group (OVX).
