Figure 5 | Scientific Reports

Figure 5

From: Biostatistics mining associated method identifies AKR1B10 enhancing hepatocellular carcinoma cell growth and degenerated by miR-383-5p

Figure 5

Knock-down AKR1B10 in HeP3B cells impairs cell proliferation and arrests cell cycle. (A) QRT-PCR assay verified the significant expression decline of AKR1B10 at mRNA status in HeP3B cells through shRNA transfection by using pGU6/Neo vectors (*P < 0.05). (B) Western-blot analysis indicated a significant decrease of AKR1B10 at protein status in HeP3B cells through shRNA transfection by using pGU6/Neo vectors. The blot was cropped and matched according to the columns from the same gel. (C) The effect of AKR1B10 on cell proliferation was determined by WST assay. The cell proliferation of HeP3B cells was significantly suppressed when AKR1B10 knocked down (*P < 0.05 for d1 and d4; **P < 0.01 for d2 and d3). (D) Representative histograms describing cell cycle profiles of HeP3B cells. The cell cycle was significantly arrested in G0/G1 phase when AKR1B10 knocked down. (E) Proportion of cells in various phases of the cell cycle. The results are means of three independent experiments_SD (**P < 0.01, *P < 0.05).

Back to article page