Figure 2

ZIKV PRVABC59 permissively infects human primary RPE cells. Human primary RPE cells were challenged with ZIKV (strain PRVABC59, PR 2015, MOI of 1) for the indicated time points; uninfected cells served as control (Mock). Control and ZIKV-infected cells were subjected to immunostaining for anti-flavivirus group antigen 4G2, and representative images show the presence of ZIKV (red) and DAPI (blue, a nuclear stain). Image magnifications 20X.