Figure 9

Pharmacological inhibition of ABCG1 attenuated ZIKV infectivity in RPE cells. Human primary RPE cells were challenged with ZIKV, and expression of ABCG1 was determined by immunostaining (A). In another set of experiments, RPE cells were pretreated with a pharmacological inhibitor of ABCG1, Benzamil (50 µM), or supplemented with cholesterol (10 µM) followed by infection with ZIKV (MOI of 1) for 48 h. ZIKV infectivity was assessed by anti-flavivirus group antigen 4G2 immunostaining (B). Results are representative of two independent experiments. Image magnifications 20X.