Figure 4
Reprogramming of permeabilized MEFs induced by EGC extracts. (A) Proliferation rate of EGC extract-treated MEFs compared with MEF extract-treated MEFs at different days. Error bars represent standard error of the mean (SEM; n = 3). (B a–d) Morphology of colonies formed following treatment of MEFs with EGC extracts. (C) Induction of alkaline phosphatase activity in MEFs after exposure to EGC extracts. (D a-b) Immunofluorescence staining of Oct4 positive colonies following exposure of MEFs to EGC extracts. Scale bars = 25 µm. (E) Quantitative PCR analysis of pluripotency marker and laminA expression in MEFs after incubation with EGC extracts. Error bars indicate SEM (n = 3). Results were normalized to GAPDH expression. *P < 0.05, versus control. (F) Q-PCR analysis of imprinted gene expression between EGC extract-treated MEFs and MEF extract-treated MEFs at 4 days after treatment. Results were normalized to human histone H2A.Z (H2AFZ) expression. *P < 0.05, **P < 0.01, versus control.
