Figure 7 | Scientific Reports

Figure 7

From: In-Vivo Nucleus Pulposus-Specific Regulation of Adult Murine Intervertebral Disc Degeneration via Wnt/Beta-Catenin Signaling

Figure 7

Suppression of b-Catenin in lumbar IVDs reduced b-Catenin and altered the expression of extracellular matrix genes. (A) Experimental design/timeline of 4 mo old bCAT cKO mice and harvest after 1 mo. Red “X” indicates tissue harvest. Letters on the right side of the “X” indicate the panels in the timeline. (B) Immunohistochemical stain for b-Catenin (brown stain, orange arrow) in the NP and (C) its quantification (no change in annulus fibrosus). (D) Gene expression of bCAT cKO versus WT discs (n = 5–6). Scale bar: (B) 25 µm. NP: nucleus pulposus. bCAT cKO vs. WT: *p < 0.05.

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