Figure 8
From: Cotton rat lung transcriptome reveals host immune response to Respiratory Syncytial Virus infection
Validating the expression analysis data. We randomly selected three up-regulated transcripts, which were unique to cotton rat-based on RNA-Seq differential expression analysis. Real-time PCR primers were designed for each transcript. qRT-PCR measured relative mRNA expression profiles of the transcripts TR453762, TR529629, and TR5333 at 6 h, 12 h, 24 h, 48 h, 4d, 5d and 7d p.i. in the RSV-infected cotton rat lung tissues. The qRT-PCR results correlate with the RNA-Seq expression profiles.
