Figure 1 | Scientific Reports

Figure 1

From: The Impact of Compressed Femtosecond Laser Pulse Durations on Neuronal Tissue Used for Two-Photon Excitation Through an Endoscope

Figure 1

Fluorescence spectra detected by the endomicroscope at 800 nm excitation and at different laser pulse durations for (a) Rhodamine B and (b) Fluorescein. Each spectrum is normalized to the fluorescence obtained at 40 fs; (c) the integrated fluorescence intensity normalized to that measured at 40 fs, while the dashed curve is the corresponding fit to a power function with exponent b. The exponent describing fluorescein’s decay function is 1.28, while that of Rhodamine B is 0.94; (d) the mean fluorescence lifetime values (ns) for each dye as a function of pulse duration (fs). Error bars correspond to the standard deviation from five measurements per pulse.

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