Table 1 Analysis of N-terminal fragments of Chs5 from different fungal origins expressed under GAL1 promoter.
Protein | Intracellular spots after 1 or 3 hours expression | Co-Localization with Sec. 7-mR2 (%) | Co-Localization with Chs6-mCh (%) | Calcofluor sensitivity | LiCl sensitivity | Hygromycin sensitivity |
|---|---|---|---|---|---|---|
ScChs5* | +++(1h) | 93.2 ± 1(1h) | 90.1 ± 6.2(1h) | ++ | + | +++ |
KlChs5* | +++(1h) | 92.4 ± 0.8(1h) | 52.6 ± 7(1h) | + | ++ | +++ |
CaChs5* | ++(1h) | 91.0 ± 0.8(1h) | None(1h) | — | ++ | +++ |
UmChs5* | +(3h) | <10(3h) | NA | — | ++ | — |
McChs5* | +(3h) | <10(3h) | NA | NA | NA | NA |
