Table 1 Thermodynamic parameters of unfolding of AcmJRL at pH 7 and 20 °C, as obtained from analysis of equilibrium transitions monitored by both intrinsic fluorescence and Far-UV CD measurements.
Technique | ΔG°(H2O)NU (kJ·mol−1) | −mNU (kJ·mol−1·M−1) | Cm (M) |
---|---|---|---|
Intrinsic fluorescence | 59 ± 4 | 9.2 ± 1.1 | 3.3 ± 0.4 |
Far-UV CD | 59 ± 2 | 9.6 ± 0.7 | 3.1 ± 0.3 |