Table 1 Thermodynamic parameters of unfolding of AcmJRL at pH 7 and 20 °C, as obtained from analysis of equilibrium transitions monitored by both intrinsic fluorescence and Far-UV CD measurements.

From: Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem

Technique

ΔG°(H2O)NU

(kJ·mol−1)

mNU

(kJ·mol−1·M−1)

Cm (M)

Intrinsic fluorescence

59 ± 4

9.2 ± 1.1

3.3 ± 0.4

Far-UV CD

59 ± 2

9.6 ± 0.7

3.1 ± 0.3