Table 2 Thermodynamic parameters of saccharide binding to AcmJRL at 25 °C for 2 equivalent binding sites (n = 2).

From: Biochemical and structural characterization of a mannose binding jacalin-related lectin with two-sugar binding sites from pineapple (Ananas comosus) stem

Saccharide

Ka (M−1)

ΔG°b (kJ mol−1)

ΔH°b (kJ mol−1)

TΔS°b (kJ mol−1)

D-Glucose

83 ± 3

−11.0 ± 0.4

−23.3 ± 0.6

−12.3

GlcNAc

88 ± 1

−11.1 ± 0.1

−25.6 ± 0.2

−15.5

D-Mannose

178 ± 4

−12.8 ± 0.3

−20.0 ± 0.3

−7.2

Manα1,3Man

421 ± 52

−15.0 ± 1.8

−14.6 ± 1.0

0.4

Mannotriose(a)

734 ± 117

−16.3 ± 2.6

−16.5 ± 1.2

−0.2

Mannopentaose(b)

1694 ± 679

−18.4 ± 6.7

−14.5 ± 2.0

3.9

  1. (a)αMan(1,3)(αMan(1,6))Man.
  2. (b)αMan(1,3) (αMan(1,6))-αMan(1,6) (αMan(1,3))Man.
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