Figure 1
Periplasmic region of FliL is required for its polar localization. (A) A schematic of polar FliL or chimeric FliL. Protein second structure is predicted using Topcons68. The α-helix labeled in a rectangular shape. β-sheet in an arrow shape. N-terminal transmembrane segment of wild-type polar FliL ends at residues of L39. Black, red, and blue colors represent segments of polar FliL, lateral FliL of V. alginolyticus and E. coli FliL respectively. TM indicates the transmembrane region. (B) Representative fluorescent microscopic images of eGFP fused to polar FliL or chimeric FliL in ZSW1 cell. Scale bar = 2 μm. (C) Detection of chimeric FliL constructs fused with eGFP, by immunoblot using anti-eGFP antibody. White triangles indicate the positions of FliL fusion constructs. Full-length blot is shown in Fig. S1.
