Figure 4
Stator-units and FliL dynamics on the flagellar motor. (A) Sample time-lapse images of fluorescent recovery after photobleaching experiments. A fluorescent spot of the assembled stator-units (green circle at time 0 s) was photobleached by a focus laser beam. The intensity of fluorescent spot region disappeared at time 0.5 (s) and then recovered by time. After about 8.5 s, there is a clear fluorescence spot in the same region. (B) The fluorescent recovery curves of three strains. Strains are the same as in Fig. 3, eGFP-Stator/+FliL (ZSW5/pHFGBA2), eGFP-Stator/ΔFliL (ZSW6/pHFGBA2), and stators/eGFP-FliL (ZSW6/pZSW81). Each color scatters with error bars represent the mean and standard error of the mean respectively from at least 25 cells. The curve was derived from fitting the theoretical recovery equation giving the turnover time48. The inset shows the turnover half-time (ln(2)/Koff) with fitting errors for different strains.
