Figure 5
From: Allosteric sodium binding cavity in GPR3: a novel player in modulation of Aβ production
βarr2 decreases constitutive and DPI-induced activity of GPR3. (A) HEK293 cells transfected with empty vector (pcDNA3.1), WT GPR3 and single point mutants were treated with DMSO or 50 μM DPI for 30 minutes and the intracellular cAMP level was measured. (B) The same experiment as in A was performed in presence of co-transfected human βarr2. 10 μM Forskolin, a known adenylate cyclase activator, was used as a positive control. Data are the mean ± SD of four independent experiments. cAMP values were normalized to the control (empty vector untreated). Statistical significance was determined by one-way ANOVA with Bonferroni-Holmes post-hoc test comparing all samples with the relative control (empty vector) (*p < 0.05, **p < 0.01).
