Figure 6
From: Allosteric sodium binding cavity in GPR3: a novel player in modulation of Aβ production
D86A mutation reduces the GPR3-stimulated Aβ production in H4swe cells. H4swe cells transfected with empty vector (pcDNA3.1), WT GPR3 and single point mutants, either in absence (blue bars) or in presence (cyan bars) of co-transfected human βarr2, were treated with DMSO (A) or 1 μM DPI (B) for 24 hours and the amount of Aβ 1–42 in the culture medium was determined by ELISA. Data are the mean ± SD of three independent experiments. Statistical significance was determined by one-way ANOVA with Bonferroni-Holmes post-hoc test comparing all samples with the relative control (empty vector) (*p < 0.05).
