Table 1 Oligonucleotide PCR primer.

From: Three newly identified Immediate Early Genes of Bovine herpesvirus 1 lack the characteristic Octamer binding motif- 1

Primer Name

Sequence

Position in Genome

UL 33/F

5′ CCCCCCGAGGCGCTGGC 3′

42413–42430

UL33/R

5′ CCCGCCCGAGCCGTGTGC 3′

42229–42246

Ul34/F

5′ CGGCCAGGACGGAAGCAACGAG 3′

41819–41840

Ul34/R

5′ CGCGGGCCTTGATCTTCTCCAGC 3′

41587–41609

Ul21/F

5′ GGGGGCGCGTTTGTGGACTG 3′

68574–68593

Ul21/R

5′ GCCCGAGAGCGCGTTGGTGAT 3′

68359–68380

BICP0/F

5′ GCCTGCATCCGCCGGTGG 3′

102578–102595

BICP0/R

5′ GGCCGCCGTGAGGTCGATGG 3′

102386–102405

BICP4/F

5′ CGGAGAGCAGCGAGGACGACGG 3′

107484–107505

BICP/R

5′ GCTTCGATGGCGGCGGCTATGA 3′

107226–107247

TK/F

5′ GGCGGGCCTGGTTGCGTACTAC 3′

63532 to 63553

TK/R

5′ GGCCGCGAGCATGAGCAGATCT 3′

63727 to 63748

gB/F

5′ CACGGACCTGGTGGACAAGAAG′

624–645

gB/R (kataria et al., 1997)

5′ CTACCGTCACGTGCTGTGTAC 3′

1070–1091

GAPDH/F

5′ CAAGGTCATCCATGACAACTTTG 3′

First Strand cDNA Synthesis

GAPDH/R

5′ GTCCACCACCCTGTTGCTGTAG 3′

Kit (#K1612) Thermo Scientific

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