Table 2 RACE Primers.
Gene | Inner Primer Sequence | Outer Primer Sequence | Position in Genome |
|---|---|---|---|
UL 21 | 5′ CGCCCCCAGCCGCGCAGCTCCAG 3′′ | 5′ CGCGCCAGGCAGTCCACAAAC 3′ | 68694–68716 (Inner primer position) 68567–68585 (outer primer sequence) |
UL33 | 5′ TCGAGCTCGCGCGGCGCCACGTC 3′ | 5′ GCCCGAGCCGTGTGCGATC 3′ | 42317–42339 (Inner primer position) 42232–42250 (Outer primer position) |
UL34 | 5′ GCGCGCCGGGGGGCCGCGAGAAG 3′ | 5′ CGCGAAAGGGCCGTGGGTCTA 3′ | 41696–41708 (Inner primer position) 41560–41580 (Outer primer position) |
RACE Primers (Provided in Kit) | 5′ CGCGGATCCGAACACTGCGTTTGCTGGCTTTGATG 3′ | 5′ GCTGATGGCGATGAATGAACACTG 3′ | Universal primers provided in the kit First Choice RLM-RACE Kit (Life technologies) |
