Figure 1
From: A Rho-based reaction-diffusion system governs cell wall patterning in metaxylem vessels
ROPGEFs and ROPGAPs regulate the secondary cell wall pit patterns. (A–C) Phenotype of metaxylem vessels in roots of wild-type (WT), ropgef4-1 (gef4-1), ropgef7-1 (gef7-1), and ropgef4-1 ropgef7-1 plants (C), of ropgef4-1 plants expressing GFP-ROPGEF4, and of ropgef7-1 plants expressing YFP-ROPGEF7 (A and B). (D–F) Phenotype of metaxylem vessels of WT, ropgap3-1, ropgap4-2, and ropgap3-1 ropgap4-2 plants (F), and ropgap3-1 ropgap4-2 plants harbouring pROPGAP4:GFP-ROPGAP4 (D and E). (G–I) Phenotype of metaxylem vessels of WT and ROPGAP3ox plants. (A,D and G) Differential interference contrast microscopy (DIC) of xylem vessels. Arrowheads indicate secondary cell wall pits. Scale bars = 10 (A and G) and 5 (D) µm. (B,E and H) Density of secondary cell wall pits in roots. Data are means ± SD (n = 10 (B) and 12 (E and H) plants). **P < 0.01; ***P < 0.001; ANOVA with Scheffe’s test (B and E) and Wilcoxon rank sum test (H). (C,F and I) Area of secondary cell wall pits in roots. Data are means ± SD (n = 10 (F) and 12 (C and I) plants). **P < 0.01; ***P < 0.001; ANOVA with Scheffe’s test (C and F) and student’s t test (I).
