Figure 1

Characterization of hiPSC line and hiPSC-CMs. Representative data from BrS hiPSCs are presented. (A) The expression of pluripotent stem cell markers Oct-4, SSEA-4, Tra-1–60 and Tra-1–81; (B) Teratoma formation in SCID mice after hiPSCs injection confirmed by identifying three primitive germ layers: intestine (top) for endoderm, cartilage (middle) for mesoderm and neuroepithelium (bottom) for ectoderm. (C) Normal karyotype identified with BrS hiPSCs. (D) markers of cardiomyocytes identified with hiPSC-CMs. Top: α-actinin; Middle: β-MHC; and Bottom: cardiac titin.