Figure 2 | Scientific Reports

Figure 2

From: Identification of an INa-dependent and Ito-mediated proarrhythmic mechanism in cardiomyocytes derived from pluripotent stem cells of a Brugada syndrome patient

Figure 2

Expression of SCN5A/Nav1.5 and INa density documented in tsa201 heterologous expression system. tsa201 cells transfected with equal amount of WT-SCN5A, A226V-SCN5A, R1629X-SCN5A and A226V-SCN5A + R1629X-SCN5A (50% each) were assayed for SCN5A/Nav1.5 expression and INa density. (Aa) Representative immunofluorescence staining images of Nav1.5 (purple) in tsa201cells. Arrows indicate the Nav1.5 staining. Levels of Nav1.5 were semi-quantified and plotted in a bar-graph (n = 3). (Ab) Representative Western blotting image of Nav1.5 in tsa201 transfected cells (n = 3). (B) Peak INa density measured in transfected tsa201 cells. (C) and (D) Steady state (SS)-inactivation and activation curves of INa measured in tsa201 expressing WT- and A226V-SCN5A. *p < 0.05; vs. WT-SCN5A.

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