Figure 5
Effect of MHM6 (anti-CD23), omalizumab (anti-IgE) and 8D6 (anti-IgE) antibodies on IgE-Fc binding to membrane CD23 (mCD23) on RPMI 8866 cells. IgE-Fc Alexa 647 was added to 0.5 × 106 RPMI 8866 cells with or without MHM6, omalizumab or 8D6 at a molar ratio of 1:2 for 30 min at 4 °C. Cells were then washed and acquired for flow cytometry analysis. (a) Histograms show the levels of IgE-Fc bound to mCD23 on RPMI 8866 cells as determined by flow cytometry. (b) Summary of the mean fluorescence intensity (MFI) made relative to IgE-Fc Alexa 647 alone (n = 6). Statistical analysis was performed using the One-Way ANOVA, with Bonferroni correction. A p value of < 0.05 was considered significant (*p < 0.05,**p < 0.01,***p < 0.001).
