Figure 6

Effect of pre-exposure to DON on the lysosomes of A431 cells. (a) Images are representative of A431 cells after incubation in static (+STATIC 24 h) or mechanically stimulated environment (+STRETCHED 24 h, 0.5 Hz, 15% deformation) cell nuclei (blue) and lysosomes (red). (b) Quantification of the integrated density of the LysoTracker/area. (c) Mean fluorescence/cell. (d) % of cellular area covered by lysosomes. Data are expressed as mean ± S.E. and describe controls (DMSO 1:1000, white graphs) and cells pre-incubated with DON (grey graphs); static incubations (solid bars) and mechanically stimulated incubations (striped bars) and result from the quantification of n ≥ 45 cells randomly chosen from 3 independent experiments. ***Indicates with Student’s t-test at 0.001 level significant increase in comparison to STATIC incubation. ^δδIndicates with Student’s t-test at 0.01 level significant decrease in comparison to STATIC incubation. ^aIndicates significant increase in comparison to STATIC CONTROLS with Student’s t-test at 0.05 level.