Figure 7

Impact of biomechanical stimulation on the toxicity of DON. (a) Merged images between bright field imaging and fluorescence imaging of the lysosomes (LysoTracker, red). (b) Quantification of LysoTracker fluorescence. Data describe controls (DMSO 1:1000, white graphs) and cells incubated with DON (grey graphs) depicting in solid bars static incubations and in striped bars the mechanically pre-conditioned cells. (c) Images of lysosomes (LysoTracker, red) and mitochondria (MitoTracker, green) and quantification of MitoTracker fluorescence. Data are average of the quantification of n ≥ 12 randomly acquired optical fields from at least 3 independent cell preparations. Data are expressed as mean ± S.E. For every treatment δ, δδ indicate at 0.05 and 0.01 level significant decrease of the signals in comparison to cells maintained in STATIC conditions (Mann-Whitney Test).