Table 2 Gene specific primer sequences and annealing temperatures for RT-qPCR.

From: Evidence supporting oxidative stress in a moderately affected area of the brain in Alzheimer’s disease

Gene

Primer Sequence

Annealing Temp (°C)

Primer efficiency value

SDHA

(F) – TGGGAACAAGAGGGCATCTG

(R) – CCACCACTGCATCAAATTCATG

65

2.10

NFE2L2 (e1-2)

(F) – CGTCCCAGCAGGACATGG

(R) – GCTCATACTCTTTCCGTCGC

65

2.00

HMOX1

(F) – ACTGCGTTCCTGCTCAACAT

(R) – GGGGCAGAATCTTGCACTTT

65

2.13

HMOX2

(F) – CACTGGCCAGAGAGACCTTG

(R) – CTCCAGGGCACCTTTCTCTT

65

2.13

SOD1

(F) – GGTGTGGCCGATGTGTCTAT

(R) – CACCTTTGCCCAAGTCATCT

65

2.08

  1. Primers were synthesized by Geneworks (Hindmarsh, South Australia) and stored at −20 °C. Prior to use the stock solutions were diluted 10x to yield a working stock solution (10 μM). Primers sequences were blast searched and confirmed to target the gene of interest in these human tissues.
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