Figure 2
From: Venom Complexity in a Pitviper Produced by Facultative Parthenogenesis
Proteomic 2D gel electrophoretic and RP-HPLC chromatographic profiles of the parthenogen, mother and unrelated snakes. (A) 2D SDS-PAGE gel electrophoresis profiles of venom from the wild-caught parthenogen mother (204F), her parthenogen male offspring (74M), and unrelated female (19F) and male (83M) individuals of the same age as the parthenogen from the same population. (B) RP-HPLC separations of venom from the same individuals. Chromatographic peaks were collected manually and analysed by SDS-PAGE (insets) under non-reduced (upper panels) and reduced (lower panels) conditions. Dashed lines in insets indicated where different SDS-PAGE gels were used to visualise the various fractions. Protein bands were excised and characterized by LC-nESI-MS/MS (Tables S1–S4).
