Figure 4

(A) Estrous cycles of control and repeated abortion mice in aging. D diestrus, E: estrus, M: metestrus, P: proestrus. Control mice in aging exhibited normal 4–6 day estrous cycles and repeated abortion mice became acyclic. n = 10. (B) Repeated abortion induced ablation of ERα expression in CA1 and CA3 subregions of hippocampus (HP). Scale bars: 100 µm. Bar chart illustrates quantification of ERα positive cells in the CA1 and CA3 subregion. (C) Repeated abortion induced ablation of ERα expression in VMH and ARC subregions of hypothalamus (HT). Scale bars: 100 µm. Bar chart illustrates quantification of ERα positive cells in VMH and ARC subregions. (D) Repeated abortion induced ERα protein expression downregulation in HP and HT in aging. x ± s. ^**P < 0.01, ^*P < 0.05 vs. Con. n = 6.