Figure 1

Localization and expression of autophagy related gene in GV (0 h) and MII (44 h) stage oocytes. (A) Relative mRNA expression levels of LC3 and BECN1 at the GV and MII stage oocytes analyzed by qRT PCR. mRNA expression at the GV stage was arbitrarily set as onefold. Fold differences in the mRNA expression from equivalent numbers of GV and MII stage embryos are shown after normalisation against the internal standard GAPDH. Data are presented as the mean ± SEM. ^*P < 0.05. (B) Oocytes at the GV and MII stage were immunolabeled with anti-LC3 antibody and Hoechst33342 to visualize the localization of LC3 in porcine oocytes. The MII polar body is indicated by a white arrow. In the enlarged panel (indicated by the white box) dots represent the localization of LC3. Scale bar = 20 μm. (C) Quantification of LC3 dots in oocytes. Each value represents the mean ± SEM.