Figure 2
From: Autophagy is required for proper meiosis of porcine oocytes maturing in vitro
BECN1 dsRNA inhibits extrusion of the first polar body. (A–C) Knockdown of endogenous BECN1 mRNA and protein expression after BECN1 dsRNA injection was verified by qRT-PCR (A) and immunofluorescent staining (B,C). BECN1 mRNA and protein expression were significantly decreased after dsRNA injection. In the enlarged panel (indicated by the white box) dots represent the localization of LC3 labelled protein, BECN1 labelled protein. Note that the colocalisation of LC3 and BECN1. Scale bar = 20 μm. Each value represents the mean ± SEM. *P < 0.01. (D) Effect of BECN1 knockdown on the rate of oocyte polar body extrusion after 44 h of in vitro culture. The MII polar body is indicated by a white arrow. Scale bar = 20 μm. Each value represents the mean ± SEM. *P < 0.05.
