Figure 4
From: Autophagy is required for proper meiosis of porcine oocytes maturing in vitro
Western blotting assay for conversion of LC3-I (cytosolic) to LC3-II (autophagosome bound). (A) LC3- I (lane 1), LC3-II (lane 2) and GAPDH (lane 3) in untreated MII (Control), dsBECN1-injected (dsBECN1) and LY294002 treated (LY294002) oocytes. Graphs (B,C) show LC3-II quantification by western blotting in oocytes in different groups. While B shows LC3-II contents (LC3-II/GAPDH ratio), (C) shows the LC3-II/ LC3- I ratio. *P < 0.05.
