Figure 5
From: Autophagy is required for proper meiosis of porcine oocytes maturing in vitro
DNA damage and apoptosis of oocytes after dsBECN1 injection or autophagy inhibitor treatment. (A) Localization of γH2A.X in the nuclei of oocytes and early apoptosis in the membrane of oocytes by performing annexin-V staining. The percentage of γH2A.X positive and annexin-V positive oocytes significantly increased after injection of dsBECN1 or LY294002 treatment. Blue, DNA; red, γH2A.X; green, Annexin-V. The MII polar body is indicated by a white arrow. Scale bar = 20 μm. (B) Quantification of γH2A.X positive oocytes. Each value represents the mean ± SEM. *P < 0.01. (C) The percentage of annexin-V-positive oocytes. Each value represents the mean ± SEM. *P < 0.01. (D) DNA damage in oocytes was assessed by performing the comet assay. Control oocytes showed slight DNA damage, whereas dsBECN1-injected or LY294002-treated oocytes showed notable DNA damage. Scale bar = 20 μm. (E) Fold changes in tail moment and length in oocytes. Each value represents the mean ± SEM. *P < 0.01.
