Table 1 Genes and specific primer sequences used in quantitative RT-PCR.
Gene symbol | Gene name | NCBI Reference Sequence | Primer set sequences (5′-3′) | Product length (nt) | Reference |
|---|---|---|---|---|---|
ACTB | actin beta | NM_173979 | F: CCTTCCTGGGCATGGAATCCT R: TCTTCATTGTGCTGGGTGCC | 186 | * |
AREG | amphiregulin | NM_001099092 | F: ACTTTGGTGAACGATGTGGGG R: TCGTCTTCGAAGCAGGATTGTA | 155 | * |
GAPDH | glyceraldehyde-3-phosphate dehydrogenase | NM_001034034 | F: TGTTGTGGATCTGACCTGCC R: TGTCGTACCAGGAAATGAGCTT | 224 | * |
HAS2 | hyaluronan synthase 2 | NM_174079 | F: TAAATGTGGCAGGCGGAAGAAGG R: GTCTTTGTTCAAGTCCCAGCAGCA | 183 | |
IL6 | interleukin 6 | NM_173923 | F: CAATCTGGGTTCAATCAGGCGAT R: GCATCTTCTCCAGCAGGTCAG | 220 | * |
LIF | leukemia inhibitory factor | NM_173931 | F: TCCTCTATTACACGGCCCAGG R: TCACGTGGTACTTGCTGCAC | 294 | * |
pri-miR-21 | primary-miR-21 | MF966934 MF966935 | F: ATGGCTGTACCACCTTGTCG R: GTGCCACTAGACCTAAGGACC | 192 | * |
STAT3 | signal transducer and activator of transcription 3 | NM_001012671 | F: CTCTCCCCACTTCTGCCAAG R: AGGGGTCACAACTGCTGCTC | 118 | * |
TNFAIP6 | TNF alpha induced protein 6 | NM_001007813 | F: TGTCCTGCTATGGGAAGAGG R: TGCTTGTAGGTGGCAAGATG | 186 | |
YWHAZ | tyrosine 3-monooygenase/tryptophan 5-monooxygenase activation protein zeta | BM446307 XM_019973801 | F: GCATCCCACAGACTATTTCC R: GCAAAGACAATGACAGACCA | 120 |
