Figure 4 | Scientific Reports

Figure 4

From: AEBP1 promotes epithelial-mesenchymal transition of gastric cancer cells by activating the NF-κB pathway and predicts poor outcome of the patients

Figure 4

Silencing AEBP1 reduces the proliferation of gastric cancer cells. (A) The knockdown efficiencies of AEBP1 in MGC803 and XN0422 were confirmed by Western blotting and qRT-PCR (B) analyses; *P < 0.05, **P < 0.01. (C) Representative images of colonies formed in sh-AEBP1 and mock MGC803 and XN0422 cells. (D) Histograms showing the number of colonies. The means ± standard deviations from three independent experiments were shown; *P < 0.05. (E) CCK-8 assays showing impaired proliferation ability of MGC803 and XN0422 cells with knockdown of AEBP1. (F) Western blotting analyses of PCNA and cyclin D1 in AEBP1-knockdown and mock cells. (G) Formation of subcutaneous xenograft tumors by mock and sh-AEBP1 cells. (H) Analysis of the weight of xenograft tumors; ***P < 0.001. The western blots were derived under the same experimental conditions from the same cell lysates; the original full-length Western blot images are shown in Supplementary Fig. S4.

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