Figure 3 | Scientific Reports

Figure 3

From: oxLDL inhibits differentiation and functional activity of osteoclasts via scavenger receptor-A mediated autophagy and cathepsin K secretion

Figure 3

oxLDL blocks the functional activities of osteoclasts. Monocytes were plated on hydroxyapatite coated plates and bone slices for a total of 21 days. (A) Hydroxyapatite coated plates showing a total resorptive inhibition in the presence of oxLDL. Magnification x10, scale bar 1 mm. (B) Quantification of resorbed areas on hydroxyapatite coated plates with ImageJ. (C) Fluorescence microscopy of acridine orange staining of mature osteoclasts cultured on tissue culture plates showing v-ATPase mediated acidification. Green and orange indicate neutral and acidic pH respectively. Magnification x40, scale bar 1 mm (D) oxLDL inhibits resorptive activity of osteoclasts on bone slices. Magnification x10, scale bar 1.5 mm. (E) Conditioned media from D was collected and analyzed for the presence of collagen type 1 fragment using a colorimetric ELISA method. n ≥ 3.

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