Figure 4 | Scientific Reports

Figure 4

From: oxLDL inhibits differentiation and functional activity of osteoclasts via scavenger receptor-A mediated autophagy and cathepsin K secretion

Figure 4

oxLDL regulates the gene expression and secretion of CatK. Osteoclasts were stimulated with different concentrations of oxLDL. (A) mRNA expression of CatK with and without oxLDL (B) CatK activity by fluorometric assay using synthetic substrate in control and oxLDL treated cells. (C) Cells treated with and without oxLDL for 3 days were lysed and equal amount of cell lysates were western blotted for CatK. (D) Conditioned media from control and oxLDL treated osteoclasts were subjected to gelatin zymography at day 8; intensity quantification in Di–iii. (E) Conditioned media were subjected to western blot analysis at day 8 and probed with the CatK antibody. (F) Conditioned media were subjected to western blot analysis at day 8 and probed with the MMP-9 antibody. Images from both zymography and western blot were captured using the VersaDoc‐3000 (Bio‐Rad Laboratories, Muenchen, Germany) and quantified using QuantityOne software (Bio‐Rad Laboratories). Error bars represent standard deviation. n ≥ 3.

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