Figure 6 | Scientific Reports

Figure 6

From: oxLDL inhibits differentiation and functional activity of osteoclasts via scavenger receptor-A mediated autophagy and cathepsin K secretion

Figure 6

oxLDL inhibits targeting of lysosomal proteins to the ruffled border through autophagy regulation. (A) Mature osteoclasts were stained with FITC-phalloidin (green) and co-stained with v-ATPase (subunit a3) (red) Magnification x40, scale bar 50 µm. White arrows indicate cytoplasmic localization of v-ATPase. (B) Mature osteoclasts were stained with FITC-phalloidin (green) and then co-stained with the lysosomal protein; LAMP2 (red) Magnification x63 scale bar 50 µm. White arrows indicate peripheral localization of LAMP2. (C) Monocytes plated on glass coverslips were grown in the presence of M-CSF and RANKL for a total of 15 days. Mature osteoclasts were stained with the lysosomal marker LC3b (in green) and then co-stained with CatK (in red). Magnification x63, zoom 4x, scale bar 15 µm. Quantification by ImageJ. (D) Cells were lysed at day 3 and equal amount of cell lysates were western blotted for p62.

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