Figure 7

Transmission electron microscopy of osteoclasts. Monocytes were plated in plastic cell culture dishes. Differentiation to osteoclasts and oxLDL treatment was performed as described. Control (A,C,E) and oxLDL treated (B,D,F) treated cells are shown. (A,B) Overviews showing the apical (top) and basal (bottom) side of the cells. Note the apical cell extensions (white arrowheads) in the control, which are mostly absent in the oxLDL treated. Electron dense, membrane surrounded compartments (B, asterisks) were predominantly observed in oxLDL treated osteoclasts. Higher magnifications of the cytoplasm (C,D; insets: overviews of the cells, white arrowheads: apical cell extensions) reveal rough endoplasmic reticulum (ER) in both samples (C,D; black arrowheads). In the oxLDL treated cells, electron dense compartments (D, asterisks) also appear as cisternae, in that case only few ER cisternae are observed. Golgi stacks appear enlarged in oxLDL treated osteoclasts (E,F). N, nucleus; (G) Golgi. Scale bars are 2 µm in (A,B) 1 µm in (C,D) 2 µm in the insets of (C,D) 0.5 µm in (E,F) n = 2.