Figure 4 | Scientific Reports

Figure 4

From: A phenotypic small-molecule screen identifies halogenated salicylanilides as inhibitors of fungal morphogenesis, biofilm formation and host cell invasion

Figure 4

Niclosamide and TCSA antivirulence activity are expanded to the inhibition of biofilm formation and host invasion. Effect of TCSA (A,C) and Niclosamide (B,D) on biofilm formation of C. albicans (A,B) and C. auris (C,D). The effect of HSA on biofilm formation of both C. albicans SC5314 and C. auris HDQ-RPCau1 strains was assessed using the metabolic colorimetric assay based on the reduction of XTT. Results represent growth inhibition (%) and are shown as the mean of at least three independent replicates. (E) Both Niclosamide and TCSA attenuate damage of enterocytes cells caused by C. albicans. Damage of the human epithelial intestinal cells HT-29 infected by C. albicans SC5314 strain was assessed using LDH release assay. Cell damage was calculated as percentage of LDH activity of HSA-treated experiment relatively to that of the control experiment (C. albicans invading HT-29 cells in the absence of HSA). Results are represented as the mean of three independent replicates.

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