Table 1 Summary of the i-GONAD procedure shown in Fig. 1C.

From: i-GONAD (improved genome-editing via oviductal nucleic acids delivery), a convenient in vivo tool to produce genome-edited rats

Treatment

No. rats treated

No. pregnant rats

No. 2-cell embryos collected

No. 2-cell embryos showing red fluorescence (%)

No. 2-cell embryos showing green fluorescence (%)

i-GONAD

3

2

20

17 (85)

9 (45)

Control (without electroporation)

2

2

17

0 (0)

ND

  1. ND: Not done.