Table 3 Summary of the offspring obtained through i-GONAD-based KI.
Experiment | Treatment | No. pregnant rats/no. rats treated | No. fetuses/pups obtained | Fetuses/pups with modified alleles | ||
|---|---|---|---|---|---|---|
No. indels (%) | No. KI allele (%) | No. mosaic alleles (%) | ||||
Exp-3 (i-GONAD using LEW strain) | Cas9/tracr/crRNA + ssODN | 5/7 | 22 | 9 (41) | 1 (5) | 2 (9) |
Exp-4 (i-GONAD using SD strain) | Cas9/tracr/crRNA + ssODN | 4/7 | 40 | 16 (40) | 2 (5) | 5 (13) |
Exp-5 (i-GONAD using SD strain) | Cas9/tracr/crRNA + ssODN + 1 μM SCR7 | 6/6 | 27 | 11 (41) | 2 (7) | 6 (22) |
Exp-5 (i-GONAD using SD strain) | Cas9/tracr/crRNA + ssODN + 10 μM SCR7 | 3/5 | 21 | 10 (48) | 2 (9) | 5 (24) |
