Figure 6

Fractionation of IMAC-purified recombinant Pr78^Gag protein by size exclusion chromatography. (a) Absorbance versus elution time chromatogram plotted from the data obtained from a Superdex 200 column showing peak fractions with maximum absorbance containing purified recombinant full-length MPMV Pr78^Gag-His₆-tag fusion protein expressed from FN1. (b) Coomassie Brilliant Blue-stained SDS-polyacrylamide gel showing the resolution of purified recombinant full-length MPMV Pr78^Gag-His₆-tag fusion protein expressed from FN1 in fractions 18–26. (c) Western blot analysis of pooled peak fractions (22–24) of purified recombinant full-length MPMV Pr78^Gag-His₆-tag fusion protein analyzed with anti-Pr78^Gag polyserum, and (d) anti-6x-His monoclonal antibody, respectively.