Figure 7
In vitro assembly by purified recombinant Pr78Gag protein to form virus like particles (VLPs). (a–f) Transmission electron micrographs showing in vitro-assembled virus like particles from purified recombinant full-length MPMV Pr78Gag-His6-tag fusion protein expressed from FN1 in the presence of yeast tRNA. (g,h) Electron micrographs of negative controls comprising of assembly buffer and yeast tRNA only without any protein. Scale bar = 50 nm; 135,000X magnification.
