Figure 4
From: Intrinsically active MEK variants are differentially regulated by proteinases and phosphatases
Effects of MEKs mutations on their phosphorylation and expression. (A) The phosphorylation and expression of MEK1 and its mutants. Cos7 and Hela cells were transfected with MEK1 and its mutants. The cells were then treated as described in Fig. 3 and blotted with anti pMEK, gMEK Abs and gERK Abs. The blotted endogenous gERK (bottom panels) is brought as a loading control. (B) The phosphorylation and expression of MEK2 and its mutants. Cos7 and Hela cells were transfected with MEK2 and its mutants. The cells were then treated as described in Fig. 3 and blotted with anti pMEK, gMEK and gERK Abs. The blotted endogenous gERK (bottom panels) is brought as a loading control. (C and D) Quantification of the results in A and B. The bar-graphs represent averages and standard errors of 3 experiments. **P < 0.05 according to student’s t-test.
