Figure 6

Chitin enhances activation of DCs dependent on IL-33. (a–c) BALB/c-wild-type mice, and (d–f) BALB/c-wild-type and -Il33^−/− mice were intranasally treated with chitin, OVA or OVA+ chitin on 3 consecutive days. Twenty-four hours after the last treatment, thoracic LNs were harvested. The proportion and number of CD45⁺ IL-1β-producing DCs in 7-AAD-negative I-A/I-E^hiCD11c^hi cells in the LNs were determined by flow cytometry. Data are pooled from 4-independent (a–c) and 2-independent (d–f) experiments and shown as the mean ± SE. 1-way ANOVA or Mann–Whitney U test, *P < 0.05.