Figure 2

(A) Cys7 and Cys22 were substituted individually and in tandem with serine and alanine to give bactofencin variants C7S, C22S, C7S-C22S, C7A, C22A, C7A-C22A. Activity (µM) of bactofencin A compared to bactofencin cysteine variants C7S, C22S, C7S-C22S, C7A, C22A and C7A-C22A is presented in (B).