Figure 1

CCNO and CNTD2 are overexpressed in human colon cancer tissues. The protein screening of atypical cyclins was evaluated by western blot analysis. (a) Representative images of the expression of atypical cyclins in human colorectal cancer cell lines, HT-29, LoVo, HT115 and HCA-7, relative to colon normal fibroblasts, CCD-18Co. (b) Representative images of the atypical cyclins expression in normal (N) and tumour (T) FFPE human colon tissues. (c) Box plots of the expression ratio between tumour and normal colon tissues (T/N ratio) is presented. The whiskers indicate the range of the data and the horizontal bars represent the median (n = 55). ***P < 0.001 vs normal tissues, Wilcoxon test. (d) Representative immunohistochemistry images of human colon carcinoma samples stained with CNTD2 and CCNO antibodies. In both cases, positive protein expression was higher in tumour tissues. CNTD2 was localized to the nucleus whereas CCNO was localized to the nucleolar region of tumour cells (magnification 63x). Western blot images (a and b) have been cropped for clarity with full blots presented in Supplementary Fig. S5