Figure 2

Overexpression of CCNO and CNTD2 increases the proliferation of CRC cells. LoVo and HT-29 cells were infected with empty lentiviral vector (control) or with the indicated cyclin-overexpressing construct. (a,d) CRC cell number was evaluated by counting. Columns represent the mean ± SEM of twelve independent experiments. **P < 0.01, ***P < 0.001 vs control, Mann-Whitney test. (b,e) The proliferation of CRC cells was evaluated by ki-67 immunostaining. Columns represent one representative experiment of four. In each experiment around 100 cells were analysed. **P < 0.01, ***P < 0.001 vs control, T-student test. (c,f) Efficiency of cell colony formation of CRC cells. Columns represent the mean ± SEM of eighteen independent experiments *P < 0.05 vs control, Mann-Whitney test. Results are expressed as a percentage of control.