Figure 3

Overexpression of CNTD2 enhances the migration of CRC cells. (a) The T/N ratio of CNTD2 was evaluated in FFPE CRC tissue samples obtained from patients with different grade of regional lymph nodes (N), N0 (n = 25), N1 (n = 18) or N2 (n = 12) and is represented as box plots. *P < 0.05, Mann-Whitney test. (b,d) The migration of CRC cells was evaluated by the wound assay and the results are expressed as wound closure in µm. The columns represent the mean ± SEM of four independent experiments performed in triplicates. *P < 0.05 vs control, Mann-Whitney test. (c,e) CRC migration was monitored by the transwell assay and quantified by measuring the absorbance at 595 nm after acetic extraction of the crystal violet. The columns represent the mean ± SEM of four independent experiments performed in triplicate. **P < 0.01 vs control, Mann-Whitney test. Results are expressed as a percentage of control.