Figure 4

Overexpression of CNTD2 promotes spheroid formation capacity and tumourigenicity in CRC cells. (a,b) Efficiency of sphere formation of CRC cells in tumour sphere medium and ultra low attachment plates. Columns represent the mean ± SEM of six independent experiments performed in triplicates. *P < 0.05, **P < 0.01 vs control, Mann-Whitney test. (c,d) Effect of CNTD2 on anchorage-independent growth was determined by soft agar assay. Columns represent the mean ± SEM of five independent experiments performed in triplicates. *P < 0.05 vs control, Mann-Whitney test. (e,f) The effect of CNTD2 overexpression on EMT markers E-cadherin and vimentin was quantitated after normalization with Ponceau. Columns represent the mean ± SEM of eight independent experiments. *P < 0.05 vs control, Mann-Whitney test. Results are expressed as a percentage of control. Western blot images (e and f) have been cropped for clarity with full blots presented in Supplementary Fig. S6.