Figure 5

CNTD2 overexpression significantly enhances tumour growth in vivo. (a,e) The tumour volumes were measured at the indicated number of days after mice were transplanted with CRC cells infected with empty vector (control; n = 7) and CNTD2-overexpressing constructs (n = 7). *P < 0.05, **P < 0.01 vs control, Mann-Whitney test. (b,f) At day 70 mice were sacrificed and the tumours were resected and weighed. *P < 0.05, vs control, Mann-Whitney test. Representative images of resected tumours obtained from empty vector (control) and CNTD2 overexpressing cell-transplanted mice are shown. Results are expressed as a percentage of control. (c,g) Representative images of immunohistochemistry analysis of Ki67 in xenografts. Scale bar: 500 μm. (d,h) The effect of CNTD2 overexpression on markers of apoptosis was monitored by western blot in resected tumours of Lovo (n = 5) and HT-29 (n = 7) cells and quantitated after normalization with Ponceau. Columns represent the mean ± SEM.*P < 0.05 vs control, Mann-Whitney test. Results are expressed as a percentage of control. Western blot images (d and h) have been cropped for clarity with full blots presented in Supplementary Fig. S7.